LOCUS       pCM62-Prha-T7SL-        8089 bp ds-DNA     circular     23-DEC-2011
ACCESSION   pCM62-Prha-T7SL-
VERSION     pCM62-Prha-T7SL-
KEYWORDS    .
FEATURES             Location/Qualifiers
     misc_feature    3334..3336
                     /label="alanine 250"
                     /vntifkey="21"
     rep_origin      948..1656
                     /label="Rep_Origin_1"
                     /vntifkey="33"
                     /note="oriV; IncP origin of replication"
     gene            5271..5936
                     /label="tetR"
                     /vntifkey="60"
     gene            complement(3966..5165)
                     /label="tetA"
                     /gene="tetA"
                     /vntifkey="60"
     gene            2587..3735
                     /label="trfA"
                     /vntifkey="60"
     oriT            1667..2405
                     /label="oriT_1"
                     /vntifkey="104"
                     /note="IncP origin of transfer"
     gene            1944..2060
                     /label="traJ'"
                     /vntifkey="60"
     CDS             1944..2060
                     /label="traJ'"
                     /gene="traJ'"
                     /codon_start="1"
                     /transl_table="11"
                     /product="TraJ'"
                     /protein_id="AAK73400.1"
                     /vntifkey="4"
                     /note="truncated oriT-recognizing protein"
                     /db_xref="GI:14794590.0"
     misc_feature    795..923
                     /label="dbl term"
                     /vntifkey="21"
     CDS             93..770
                     /label="RFP"
                     /vntifkey="4"
     terminator      5973..6078
                     /label="T0"
                     /vntifkey="43"
     promoter        7855..91
                     /label="PrhaB"
                     /vntifkey="30"
     CDS             7021..7854
                     /label="rhaS"
                     /vntifkey="4"
     CDS             6099..7118
                     /label="rhaR"
                     /vntifkey="4"
     misc_feature    28..66
                     /label="T7 stem loop"
                     /vntifkey="21"
     misc_feature    29
                     /label="trascriptional start?"
                     /vntifkey="21"
ORIGIN      
        1 gaattcaggc gctttttaga ctggtcgtag ggagaccaca acggtttccc tctagaaata
       61 attttgagat cttttaagaa ggagatatac atatggcgag tagcgaagac gttatcaaag
      121 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg
      181 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca
      241 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca
      301 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag
      361 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg
      421 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc
      481 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta
      541 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
      601 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
      661 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
      721 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa ggatccaaac
      781 tcgagtaagg atctccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      901 gggtgggcct ttctgcgttt atacctaggc agaagtggtc agcttggctg ccatttttgg
      961 ggtgaggccg ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc
     1021 gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca
     1081 gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga
     1141 caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg
     1201 tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa
     1261 gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca
     1321 gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt
     1381 tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct
     1441 catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc
     1501 tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg
     1561 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag
     1621 cggcgagggc aaccagcccg gtgagcgtcg gaaagggtcg acggatcttt tccgctgcat
     1681 aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat
     1741 atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc
     1801 cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt
     1861 attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg gctaccgccg
     1921 gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg aagggcagcc
     1981 cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa aaggcggcgg
     2041 cggccggcat gagcctgtag gcctacctgc tggccgtcgg ccagggctac aaaatcacgg
     2101 gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac ctgggccgcc
     2161 tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg ttcggtgatg
     2221 ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt ggcaaggtca
     2281 tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta aaacggccgg
     2341 ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca tcaagaagag cgacttcgcg
     2401 gagctggtat tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg
     2461 gtctacggga ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc
     2521 gggtcaaatc aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga
     2581 gggtgaatga atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg
     2641 ttttccgccg aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa
     2701 accttccagt ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc
     2761 gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt
     2821 ctcgaacagg aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg
     2881 acgaccaaga agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag
     2941 caggccgcgt tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc
     3001 gatattgcgc cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc
     3061 ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc
     3121 cacgtcaaca aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac
     3181 gaactggtgt ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc
     3241 accttcacgt tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac
     3301 acgaaggccg aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac
     3361 cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc
     3421 aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc
     3481 gaccactaca cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg
     3541 atgttcgact atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc
     3601 ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc
     3661 tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg
     3721 cattgcaaac gctagggcct tgtggggtca gttccggctg ggggttcagc agccagcgct
     3781 ttactggcat ttcaggaaca agcgggcact gctcgacgca cttgcttcgc tcagtatcgc
     3841 tcgggacgca cggcgcgctc tacgaactgc cgataaacag aggattaaaa ttgacaattc
     3901 tagggcgcgt atagcttgcc ggaagtcgcc ttgacccgca tggcataggc ctatcgtttc
     3961 cacgatcagc gatcggctcg ttgccctgcg ccgctccaaa gcccgcgacg cagcgccggc
     4021 aggcagagca agtagagggc agcgcctgca atccatgccc acccgttcca cgttgttata
     4081 gaagccgcat agatcgccgt gaagaggagg ggtccgacga tcgaggtcag gctggtgagc
     4141 gccgccagtg agccttgcag ctgcccctga cgttcctcat ccacctgcct ggacaacatt
     4201 gcttgcagcg ccggcattcc gatgccaccc gaagcaagca ggaccatgat cgggaacgcc
     4261 atccatcccc gtgtcgcgaa ggcaagcagg atgtagcctg tgccgtcggc aatcattccg
     4321 agcatgagtg cccgcctttc gccgagccgg gcggctacag ggccggtgat cattgcctgg
     4381 gcgagtgaat gcagaatgcc aaatgcggca agcgaaatgc cgatcgtggt cgcgtcccag
     4441 tgaaagcgat cctcgccgaa aatgacccaa agcgcggccg gcacctgtcc gacaagttgc
     4501 atgatgaaga agaccgccat cagggcggcg acgacggtca tgccccgggc ccaccggaac
     4561 gaagcgagcg ggttgagagc ctcccggcgt aacggccggc gttcgccttt gtgcgactcc
     4621 ggcaaaagga aacagcccgt caggaaattg aggccgttca aggctgccgc ggcgaagaac
     4681 ggagcgtggg gggagaaacc gcccatcagc ccaccgagca caggtcccgc gaccatcccg
     4741 aacccgaaac aggcgctcat gaagccgaag tgccgcgcgc gctcatcgcc atcagtgata
     4801 tcggcaatat aagcgccggc taccgcccca gtcgccccgg tgatgccggc cacgatccgc
     4861 ccgatataga gaacccaaag gaaaggcgct gtcgccatga tggcgtagtc gacagtggcg
     4921 ccggccagcg agacgagcaa gattggccgc cgcccgaaac gatccgacag cgcgcccagc
     4981 acaggtgcgc aggcaaattg caccaacgca tacagcgcca gcagaatgcc atagtgggcg
     5041 gtgacgtcgt tcgagtgaac cagatcgcgc aggaggcccg gcagcaccgg cataatcagg
     5101 ccgatgccga cagcgtcgag cgcgacagtg ctcagaatta cgatcagggg tatgttgggt
     5161 ttcacgtctg gcctccggac cagcctccgc tggtccgatt gaacgcgcgg attctttatc
     5221 actgataagt tggtggacat attatgttta tcagtgataa agtgtcaatc atgacaaagt
     5281 tgcagccgaa tacagtgatc cgtgccgccc tggacctgtt gaacgaggtc ggcgtagacg
     5341 gtctgacgac acgcaaactg gcggaacggt tgggggttca gcagccggcg ctttactggc
     5401 acttcaggaa caagcgggcg ctgctcgacg cactggccga agccatgctg gcggagaatc
     5461 atacgcattc ggtgccgaga gccgacgacg actggcgctc atttctgatc gggaatgccc
     5521 gcagcttcag gcaggcgctg ctcgcctacc gcgatggcgc gcgcatccat gccggcacgc
     5581 gaccgggcgc accgcagatg gaaacggccg acgcgcagct tcgcttcctc tgcgaggcgg
     5641 gtttttcggc cggggacgcc gtcaatgcgc tgatgacaat cagctacttc actgttgggg
     5701 ccgtgcttga ggagcaggcc ggcgacagcg atgccggcga gcgcggcggc accgttgaac
     5761 aggctccgct ctcgccgctg ttgcgggccg cgatagacgc cttcgacgaa gccggtccgg
     5821 acgcagcgtt cgagcaggga ctcgcggtga ttgtcgatgg attggcgaaa aggaggctcg
     5881 ttgtcaggaa cgttgaagga ccgagaaagg gtgctaagaa accattatta tcatgacatt
     5941 aacctataaa aataggcgta tcacgaacta gtgcttggat tctcaccaat aaaaaacgcc
     6001 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     6061 atcaacagga gtccaagcga gctcggcatg gcgaattaat ctttctgcga attgagatga
     6121 cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa atagttacta
     6181 tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg ctggagaaga
     6241 tattgcgcat gacacactct gacctgtcgc agatattgat tgatggtcat tccagtctgc
     6301 tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca aaatttatcc
     6361 agcgcaaagg gacttttcag gctagccgcc agccgggtaa tcagcttatc cagcaacgtt
     6421 tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag caacatcacc
     6481 aactgcccga acagcaactc agccatttcg ttagcaaacg gcacatgctg actactttca
     6541 tgctcaagct gaccgataac ctgccgcgcc tgcgccatcc ccatgctacc taagcgccag
     6601 tgtggttgcc ctgcgctggc gttaaatccc ggaatcgccc cctgccagtc aagattcagc
     6661 ttcagacgct ccgggcaata aataatattc tgcaaaacca gatcgttaac ggaagcgtag
     6721 gagtgtttat cgtcagcatg aatgtaaaag agatcgccac gggtaatgcg ataagggcga
     6781 tcgttgagta catgcaggcc attaccgcgc cagacaatca ccagctcaca aaaatcatgt
     6841 gtatgttcag caaagacatc ttgcggataa cggtcagcca cagcgactgc ctgctggtcg
     6901 ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc tacctcggcc
     6961 agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag attcgcgtta
     7021 ttgcagaaag ccatcccgtc cctggcgaat atcacgcggt gaccagttaa actctcggcg
     7081 aaaaagcgtc gaaaagtggt tactgtcgct gaatccacag cgataggcga tgtcagtaac
     7141 gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc ggttcaggta
     7201 tcgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac gcagtgaaag
     7261 agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt cctccagcca
     7321 ggccagaagc aagttgagac gtgatgcgct gttttccagg ttctcctgca aactgctttt
     7381 acgcagcaag agcagtaatt gcataaacaa gatctcgcga ctggcggtcg agggtaaatc
     7441 attttcccct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct gcaatacgct
     7501 gtggttaacg cgccagtgag acggatactg cccatccagc tcttgtggca gcaactgatt
     7561 cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattgg tcagacacag
     7621 attatcggta tgttcataca gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc
     7681 ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt cgacaatcac
     7741 aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc ggggttctat
     7801 cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg tcatactggc
     7861 ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt accttaaatt
     7921 ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagcgtga attttcagga
     7981 aatgcggtga gcatcacatc accacaattc agcaaattgt gaacatcatc acgttcatct
     8041 ttccctggtt gccaatggcc cattttcctg tcagtaacga gaaggtcgc 
//
