LOCUS       pGG_00005               5687 bp    dna     circular UNK 
ACCESSION   pJWT00001
KEYWORDS    ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; .
FEATURES             Location/Qualifiers
     CDS             1..2
                     /vntifkey="4"
                     /ApEinfo_fwdcolor=#00ffff
                     /label=AmpR
                     /ApEinfo_revcolor=#00ffff
     misc_marker     138..434
                     /vntifkey="22"
                     /ApEinfo_fwdcolor=pink
                     /label=Kan/neoR 5prime
                     /ApEinfo_revcolor=pink
                     /note="encodes nptII (aka AphA, neoR), gives kan and neo
                     resistance, with native promoter in on AatII and SpeI
                     from Lutz vector pZE2PLtetO-1 MCS2"
     CDS             483..1565
                     /vntifkey="4"
                     /ApEinfo_fwdcolor=pink
                     /label=lacI
                     /ApEinfo_revcolor=pink
                     /note="lacI does not contain internal EcoRV site"
     promoter        1934..2009
                     /vntifkey="30"
                     /ApEinfo_fwdcolor=pink
                     /label=lacUV5 promoter
                     /ApEinfo_revcolor=pink
     misc_binding    1950..2013
                     /vntifkey="20"
                     /ApEinfo_fwdcolor=pink
                     /label=lac operator
                     /ApEinfo_revcolor=pink
     RBS             2029..2048
                     /vntifkey="32"
                     /ApEinfo_fwdcolor=pink
                     /label=RBS
                     /ApEinfo_revcolor=pink
     CDS             2049..2726
                     /vntifkey="4"
                     /ApEinfo_fwdcolor=pink
                     /label=RFP
                     /ApEinfo_revcolor=pink
     terminator      2751..2784
                     /label=Bba_1000_Term
     CDS             complement(2881..3414)
                     /label=aacC1
     misc_marker     3706..3967
                     /vntifkey="22"
                     /ApEinfo_fwdcolor=pink
                     /label=Kan/neoR 3prime
                     /ApEinfo_revcolor=pink
                     /note="encodes nptII (aka AphA, neoR), gives kan and neo
                     resistance, with native promoter in on AatII and SpeI
                     from Lutz vector pZE2PLtetO-1 MCS2"
     rep_origin      4011..4693
                     /vntifkey="33"
                     /ApEinfo_fwdcolor=pink
                     /label=colE1
                     /ApEinfo_revcolor=pink
     terminator      4699..4804
                     /vntifkey="43"
                     /ApEinfo_fwdcolor=pink
                     /label=T0
                     /ApEinfo_revcolor=pink
     CDS             4834..5687
                     /vntifkey="4"
                     /ApEinfo_fwdcolor=#00ffff
                     /label=AmpR
                     /ApEinfo_revcolor=#00ffff
     primer_bind     4850..4869
                     /ApEinfo_fwdcolor=#996633
                     /label=RDR00097
                     /ApEinfo_revcolor=#996633
     misc_binding    4969..4974
                     /ApEinfo_fwdcolor=#00ff00
                     /label=BsaI_site
                     /ApEinfo_revcolor=#00ff00
     variation       4970..4970
                     /ApEinfo_fwdcolor=#ff0000
                     /label=AtoG_mutation
                     /ApEinfo_revcolor=#ff0000
ORIGIN      
        1 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
       61 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      121 aaagtgccac ccccgggatg attgaacaag atggattgca cgcaggttct ccggccgctt
      181 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      241 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
      301 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
      361 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
      421 gcgaagtgcc gggggacgtc ggtgcctaat gagtgagcta acttacatta attgcgttgc
      481 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      541 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
      601 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
      661 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
      721 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc
      781 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
      841 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
      901 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
      961 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
     1021 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
     1081 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
     1141 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
     1201 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
     1261 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
     1321 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
     1381 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
     1441 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
     1501 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
     1561 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
     1621 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat
     1681 taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc
     1741 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     1801 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     1861 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     1921 gtagaggatc gagatcgttt aggcacccca ggctttacac tttatgcttc cggctcgtat
     1981 aatgtgtgga attgtgagcg gataacaatt tcagaattca aaagatcttt taagaaggag
     2041 atatacatat ggcgagtagc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
     2101 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
     2161 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
     2221 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
     2281 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
     2341 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
     2401 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
     2461 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
     2521 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
     2581 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
     2641 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
     2701 aaggtcgtca ctccaccggt gcttaaggat ccaaactcga gtaaggatct cgcaaaaaac
     2761 cccgcttcgg cggggttttt tcgccctagg gcgttcggct gcggcgagcg gtatcagctc
     2821 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2881 ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt
     2941 tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc
     3001 accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct
     3061 ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa
     3121 cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag
     3181 cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg
     3241 ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga
     3301 tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa
     3361 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc
     3421 tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag
     3481 gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac
     3541 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt
     3601 acagtttacg aaccgaacag gcttatgtca attcgcatga ccaaaatccc ttaacgtgag
     3661 ttttcgttcc actgagcgtc agaccccgta gaaaagatcg caaccgcccg acggcgagga
     3721 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     3781 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     3841 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     3901 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     3961 cttctgaccc ggggagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4021 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4201 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4441 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     4501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4621 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4681 attttggtca tgactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     4741 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     4801 aagcgagctc gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4861 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4921 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcgg gacccacgct
     4981 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5041 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5101 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5161 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5221 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5281 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5341 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5401 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5461 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5521 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5581 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5641 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactc
//
