LOCUS       pJWT00001               5648 bp ds-DNA     circular     23-DEC-2011
ACCESSION   pJWT00001
VERSION     pJWT00001
KEYWORDS    .
FEATURES             Location/Qualifiers
     CDS             1..2
                     /label="AmpR"
                     /vntifkey="4"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
     misc_marker     138..434
                     /label="Kan/neoR 5prime"
                     /vntifkey="22"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /note="encodes nptII (aka AphA, neoR), gives kan and neo
                     resistance, with native promoter in on AatII and SpeI
                     from Lutz vector pZE2PLtetO-1 MCS2"
     CDS             483..1565
                     /label="lacI"
                     /vntifkey="4"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /note="lacI does not contain internal EcoRV site"
     promoter        1934..2009
                     /label="lacUV5 promoter"
                     /vntifkey="30"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     misc_binding    1950..2013
                     /label="lac operator"
                     /vntifkey="20"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     RBS             2029..2048
                     /label="RBS"
                     /vntifkey="32"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     CDS             2049..2726
                     /label="RFP"
                     /vntifkey="4"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     misc_marker     2881..3540
                     /label="CmR"
                     /vntifkey="22"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     misc_marker     3667..3928
                     /label="Kan/neoR 3prime"
                     /vntifkey="22"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /note="encodes nptII (aka AphA, neoR), gives kan and neo
                     resistance, with native promoter in on AatII and SpeI
                     from Lutz vector pZE2PLtetO-1 MCS2"
     rep_origin      3972..4654
                     /label="colE1"
                     /vntifkey="33"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     terminator      4660..4765
                     /label="T0"
                     /vntifkey="43"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
     CDS             4795..5648
                     /label="AmpR"
                     /vntifkey="4"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
     primer_bind     4811..4830
                     /label="RDR00097"
                     /ApEinfo_fwdcolor="#996633"
                     /ApEinfo_revcolor="#996633"
     misc_binding    4930..4935
                     /label="BsaI_site"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
     variation       4931
                     /label="AtoG_mutation"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
     terminator      2751..2784
                     /label="Bba_1000_Term"
ORIGIN      
        1 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
       61 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      121 aaagtgccac ccccgggatg attgaacaag atggattgca cgcaggttct ccggccgctt
      181 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      241 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
      301 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
      361 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
      421 gcgaagtgcc gggggacgtc ggtgcctaat gagtgagcta acttacatta attgcgttgc
      481 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      541 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
      601 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
      661 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
      721 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc
      781 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
      841 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
      901 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
      961 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
     1021 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
     1081 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
     1141 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
     1201 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
     1261 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
     1321 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
     1381 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
     1441 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
     1501 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
     1561 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
     1621 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat
     1681 taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc
     1741 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     1801 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     1861 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     1921 gtagaggatc gagatcgttt aggcacccca ggctttacac tttatgcttc cggctcgtat
     1981 aatgtgtgga attgtgagcg gataacaatt tcagaattca aaagatcttt taagaaggag
     2041 atatacatat ggcgagtagc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
     2101 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
     2161 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
     2221 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
     2281 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
     2341 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
     2401 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
     2461 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
     2521 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
     2581 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
     2641 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
     2701 aaggtcgtca ctccaccggt gcttaaggat ccaaactcga gtaaggatct cgcaaaaaac
     2761 cccgcttcgg cggggttttt tcgccctagg gcgttcggct gcggcgagcg gtatcagctc
     2821 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2881 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     2941 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     3001 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     3061 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     3121 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     3181 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     3241 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     3301 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     3361 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     3421 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     3481 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     3541 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     3601 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
     3661 gatatcgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     3721 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     3781 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     3841 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     3901 ctatcgcctt cttgacgagt tcttctgacc cggggagcaa aaggccagca aaaggccagg
     3961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     4441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4621 aacgaaaact cacgttaagg gattttggtc atgactagtg cttggattct caccaataaa
     4681 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     4741 tggatctatc aacaggagtc caagcgagct cgtaaacttg gtctgacagt taccaatgct
     4801 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4861 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4921 tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4981 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5041 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5101 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5161 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5221 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5281 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5341 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5401 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5461 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5521 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5581 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5641 gaatactc     
//
