LOCUS       pj5_00002               5422 bp    dna     circular UNK 
ACCESSION   unknown
FEATURES             Location/Qualifiers
     CDS             complement(7..885)
                     /label=araC
     protein_bind    914..931
                     /label=operator\O2
     promoter        complement(1036..1064)
                     /label=araC\promoter
     protein_bind    1072..1093
                     /label=operator\O1
     misc_binding    1115..1128
                     /label=CAP\site
     protein_bind    1124..1162
                     /label=Operator\I2\and\I1
     promoter        1161..1188
                     /label=pBAD\promoter
     RBS             1216..1235
                     /label=RBS
     CDS             1236..2141
                     /label=GFPuv
     misc_feature    1236..1355
                     /vntifkey="21"
                     /label=umuD\Lon\tag
     misc_feature    1356..1361
                     /vntifkey="21"
                     /label=Gly\Gly\linker
     misc_feature    1784..1784
                     /label=XhoI_silent_mutation
     misc_feature    1883..1883
                     /label=BamHI_silent_mutation
     CDS             2076..2138
                     /vntifkey="4"
                     /label=signal_peptide
     terminator      2157..2285
                     /label=dbl\term
     rep_origin      complement(2287..4515)
                     /label=pSC101**
     terminator      4516..4621
                     /label=T0
     misc_marker     complement(4637..5296)
                     /label=CmR
ORIGIN      
        1 gacgtcttat gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa
       61 ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc
      121 aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt
      181 cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg
      241 gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc
      301 aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc
      361 catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag
      421 cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg
      481 cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt
      541 ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac
      601 ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg
      661 cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac
      721 cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat
      781 aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt
      841 aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc
      901 gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt
      961 ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca
     1021 aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa
     1081 agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat
     1141 aagattagcg gattctacct gacgcttttt atcgcaactc tctactgttt ctccataccc
     1201 gtttttttgg gaatttttaa gaaggagata tacatatgtt gtttatcaag cctgcggatc
     1261 tccgcgaaat tgtgactttt ccgctattta gcgatcttgt tcagtgtggc tttccttcac
     1321 cggcagcaga ttacgttgaa cagcgcatcg atctgggtgg cagtaaagga gaagaacttt
     1381 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     1441 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
     1501 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc tcttatggtg
     1561 ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc aagagtgcca
     1621 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg aactacaaga
     1681 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaaaggta
     1741 ttgattttaa agaagatgga aacattctcg gacacaaact cgaatacaac tataactcac
     1801 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc
     1861 gccacaacat tgaagatgga tctgttcaac tagcagacca ttatcaacaa aatactccaa
     1921 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgcccttt
     1981 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg
     2041 ggattacaca tggcatggat gagctcggcg gcggcggcag caaggtctac ggcaaggaac
     2101 agtttttgcg gatgcgccag agcatgttcc ccgatcgcta aatcgagtaa ggatctccag
     2161 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2221 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2281 ttatacctag ggtacgggtt ttgctgcccg caaacgggct gttctggtgt tgctagtttg
     2341 ttatcagaat cgcagatccg gcttcagccg gtttgccggc tgaaagcgct atttcttcca
     2401 gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg tcggcagctt
     2461 tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt gagctgtaac
     2521 aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg tttcacctgt
     2581 tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat ggtgaacagc
     2641 tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta caccgttttc
     2701 atctgtgcat atggacagtt ttccctttga tatgtaacgg tgaacagttg ttctactttt
     2761 gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct cagatccttc
     2821 cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg
     2881 aaccattgag atcatactta ctttgcatgt cactcaaaaa ttttgcctca aaactggtga
     2941 gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta tgtaggtagg
     3001 aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg ttgttctcaa
     3061 gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg
     3121 ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa tctttactta
     3181 ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt tcaagcatta
     3241 acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt ttcttttgtg
     3301 ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca aaagacttaa
     3361 catgttccag attatatttt atgaattttt ttaactggaa aagataaggc aatatctctt
     3421 cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc cactggaaaa
     3481 tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc agctctctgg
     3541 ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc tgagcgtatt
     3601 ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc gtggggttga
     3661 gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca tagcgactaa
     3721 tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat tggtctaggt
     3781 gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta gtccttttcc
     3841 cgggtgatct gggtatctgt aaattctgct agacctttgc tggaaaactt gtaaattctg
     3901 ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat attcaagtgg
     3961 ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat cccagccctg
     4021 tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg caaacgctgt
     4081 ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct
     4141 cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt
     4201 tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac
     4261 tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc
     4321 acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct
     4381 gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag
     4441 gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag gcttacccgt
     4501 cttactgtcc ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     4561 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     4621 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     4681 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     4741 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     4801 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     4861 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     4921 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     4981 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     5041 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     5101 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     5161 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     5221 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     5281 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     5341 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     5401 cgtctcattt tcgccagata tc
//
