LOCUS       pj5_00003               5365 bp    dna     circular UNK 
ACCESSION   unknown
FEATURES             Location/Qualifiers
     CDS             complement(25..903)
                     /label=araC
     protein_bind    932..949
                     /label=operator\O2
     promoter        complement(1054..1082)
                     /label=araC\promoter
     protein_bind    1090..1111
                     /label=operator\O1
     misc_binding    1133..1146
                     /label=CAP\site
     protein_bind    1142..1180
                     /label=Operator\I2\and\I1
     promoter        1179..1206
                     /label=pBAD\promoter
     RBS             1234..1253
                     /label=RBS
     CDS             1254..2102
                     /vntifkey="4"
                     /label=GFPuv
     misc_feature    1257..1307
                     /label=Clostridium_BMC_sig_pep
     misc_feature    1308..1355
                     /label=gly_ser_linker
     misc_feature    1775..1775
                     /label=XhoI_silent_mutation
     misc_feature    1874..1874
                     /label=BamHI_silent_mutation
     misc_feature    2067..2099
                     /vntifkey="21"
                     /label=ssrA\tag
     terminator      2118..2246
                     /label=dbl\term
     rep_origin      complement(2248..4476)
                     /label=pSC101**
     terminator      4477..4582
                     /label=T0
     misc_marker     complement(4598..5257)
                     /label=CmR
ORIGIN      
        1 tcattttcgc cagatatcga cgtcttatga caacttgacg gctacatcat tcactttttc
       61 ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga
      121 gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt
      181 ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct
      241 aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg
      301 tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc
      361 ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg
      421 cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg
      481 cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg
      541 gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc
      601 gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta
      661 gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg
      721 tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat
      781 tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc
      841 cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc
      901 catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca
      961 ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat
     1021 taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt
     1081 ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc
     1141 catagcattt ttatccataa gattagcgga ttctacctga cgctttttat cgcaactctc
     1201 tactgtttct ccatacccgt ttttttggga atttttaaga aggagatata catatggaaa
     1261 ataacgcttt attagaacaa ataatcaatg aagttttaaa aaatatgggt ggcagtggta
     1321 gcgggagctc gggtggctca ggctctggtt ccagtaaagg agaagaactt ttcactggag
     1381 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     1441 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     1501 gaaaactacc tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct
     1561 tttcccgtta tccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
     1621 gttatgtaca ggaacgcact atatctttca aagatgacgg gaactacaag acgcgtgctg
     1681 aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaaaggt attgatttta
     1741 aagaagatgg aaacattctc ggacacaaac tcgaatacaa ctataactca cacaatgtat
     1801 acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaaca
     1861 ttgaagatgg atctgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     1921 gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgccctt tcgaaagatc
     1981 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
     2041 atggcatgga tgagctcggc ggcggcgcgg cgaacgatga aaactatgcg ctggcggcgt
     2101 aaatcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2161 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2221 ttcgggtggg cctttctgcg tttataccta gggtacgggt tttgctgccc gcaaacgggc
     2281 tgttctggtg ttgctagttt gttatcagaa tcgcagatcc ggcttcagcc ggtttgccgg
     2341 ctgaaagcgc tatttcttcc agaattgcca tgattttttc cccacgggag gcgtcactgg
     2401 ctcccgtgtt gtcggcagct ttgattcgat aagcagcatc gcctgtttca ggctgtctat
     2461 gtgtgactgt tgagctgtaa caagttgtct caggtgttca atttcatgtt ctagttgctt
     2521 tgttttactg gtttcacctg ttctattagg tgttacatgc tgttcatctg ttacattgtc
     2581 gatctgttca tggtgaacag ctttgaatgc accaaaaact cgtaaaagct ctgatgtatc
     2641 tatctttttt acaccgtttt catctgtgca tatggacagt tttccctttg atatgtaacg
     2701 gtgaacagtt gttctacttt tgtttgttag tcttgatgct tcactgatag atacaagagc
     2761 cataagaacc tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt
     2821 tttgcgtgag ccatgagaac gaaccattga gatcatactt actttgcatg tcactcaaaa
     2881 attttgcctc aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc
     2941 ttagtccgtt atgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca
     3001 tttttatctg gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt
     3061 ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt
     3121 aagtgtttaa atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat
     3181 ggtagttatt ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg
     3241 ccttgtgagt tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt
     3301 atttgttttc aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga
     3361 aaagataagg caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg
     3421 catagtttgt ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag
     3481 ttctcgtcat cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga
     3541 tgttcatcat ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag
     3601 ggttttcaat cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct
     3661 ccgttaagtc atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca
     3721 tacatctcaa ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat
     3781 gataattact agtccttttc ccgggtgatc tgggtatctg taaattctgc tagacctttg
     3841 ctggaaaact tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt
     3901 tttttgttta tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa
     3961 aaagaataga tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca
     4021 aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct
     4081 taagtagcac cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc
     4141 aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca
     4201 gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc
     4261 ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt
     4321 ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca
     4381 cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt
     4441 atcatcaaca ggcttacccg tcttactgtc cctagtgctt ggattctcac caataaaaaa
     4501 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     4561 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     4621 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     4681 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     4741 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     4801 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     4861 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     4921 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     4981 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     5041 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     5101 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     5161 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     5221 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     5281 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     5341 aacctcttac gtgccgatca acgtc
//
