LOCUS               pBbB2a_GFP        4035 bp ds-DNA   circular     20-DEC-2010
KEYWORDS    .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     RBS             722..741
                     /vntifkey="32"
                     /label=RBS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    1483..1611
                     /vntifkey="21"
                     /label=dbl term
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     CDS             742..1458
                     /vntifkey="4"
                     /label=GFP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     rep_origin      complement(1618..2908)
                     /vntifkey="33"
                     /note="From pMBIS, introduced point mutation to increase
                     copy number as described in Tao et al construct pDCQ318M2"
                     /label=BBR1 ori
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_marker     complement(3050..3905)
                     /vntifkey="22"
                     /label=Amp
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     terminator      2915..3020
                     /vntifkey="43"
                     /label=T0
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     CDS             complement(7..633)
                     /vntifkey="4"
                     /label=tetR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     promoter        634..706
                     /vntifkey="30"
                     /label=Tet promoter region
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     protein_bind    655..673
                     /vntifkey="31"
                     /label=Q1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     protein_bind    685..701
                     /vntifkey="31"
                     /label=Q2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
ORIGIN
        1 gacgtcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcaaaagatc
      721 ttttaagaag gagatataca tatgagtaaa ggagaagaac ttttcactgg agttgtccca
      781 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      841 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      901 cctgttccgt ggccaacact tgtcactact ttctcttatg gtgttcaatg cttttcccgt
      961 tatccggatc acatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     1021 caggaacgca ctatatcttt caaagatgac gggaactaca agacgcgtgc tgaagtcaag
     1081 tttgaaggtg atacccttgt taatcgtatc gagttaaaag gtattgattt taaagaagat
     1141 ggaaacattc tcggacacaa actggagtac aactataact cacacaatgt atacatcacg
     1201 gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa cattgaagat
     1261 ggctccgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     1321 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     1381 aagcgtgacc acatggtcct tcttgagttt gtaactgctg ctgggattac acatggcatg
     1441 gatgagctct acaaataagg atccaaactc gagtaaggat ctccaggcat caaataaaac
     1501 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1561 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat acctaggcta
     1621 cagccgatag tctggaacag cgcacttacg ggttgctgcg caacccaagt gctaccggcg
     1681 cggcagcgtg acccgtgtcg gcggctccaa cggctcgcca tcgtccagaa aacacggctc
     1741 atcgggcatc ggcaggcgct gctgcccgcg ccgttcccat tcctccgttt cggtcaaggc
     1801 tggcaggtct ggttccatgc ccggaatgcc gggctggctg ggcggctcct cgccggggcc
     1861 ggtcggtagt tgctgctcgc ccggatacag ggtcgggatg cggcgcaggt cgccatgccc
     1921 caacagcgat tcgtcctggt cgtcgtgatc aaccaccacg gcggcactga acaccgacag
     1981 gcgcaactgg tcgcggggct ggccccacgc cacgcggtca ttgaccacgt aggccaacac
     2041 ggtgccgggg ccgttgagct tcacgacgga gatccagcgc tcggccacca agtccttgac
     2101 tgcgtattgg accgtccgca aagaacgtcc gatgagcttg gaaagtgtct tctggctgac
     2161 caccacggcg ttctggtggc ccatctgcgc cacgaggtga tgcagcagca ttgccgccgt
     2221 gggtttcctc gcaataagcc cggcccacgc ctcatgcgct ttgcgttccg tttgcaccca
     2281 gtgaccgggc ttgttcttgg cttgaatgcc gatttctctg gactgcgtgg ccatgcttat
     2341 ctccatgcgg taggggtgcc gcacggttgc ggcaccatgc gcaatcagct gcaacttttc
     2401 ggcagcgcga caacaattat gcgttgcgta aaagtggcag tcaattacag attttcttta
     2461 acctacgcaa tgagctattg cggggggtgc cgcaatgagc tgttgcgtac cccccttttt
     2521 taagttgttg atttttaagt ctttcgcatt tcgccctata tctagttctt tggtgcccaa
     2581 agaagggcac ccctgcgggg ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa
     2641 gtggcccctc cggggcttgt tgatcgactg cgcggccttc ggccttgccc aaggtggcgc
     2701 tgcccccttg gaacccccgc actcgccgcc gtgaggctcg gggggcaggc gggcgggctt
     2761 cgcccttcga ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt caaggccaag
     2821 ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc aaccggcaag
     2881 cgaagcgcgc aggccgcagg ccggaggcac tagtgcttgg attctcacca ataaaaaacg
     2941 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     3001 ctatcaacag gagtccaagc gagctcgtaa acttggtctg acagttacca atgcttaatc
     3061 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3121 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3181 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3241 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3301 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3361 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3421 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3481 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3541 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3601 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3661 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3721 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3781 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3841 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3901 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3961 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4021 cgaaaagtgc cacct    
//
