As noted in the following j5 installation documentation page, users will not need to install j5 on their own computer, since a web interface for the software is accessible at: https://j5.jbei.org/
In addition, the j5 XML-RPC web-service allows users to utilize the full functionality of j5 through locally installed XML-RPC thin client software, without having to install and maintain j5 on their own machines.
There are several software packages that are required for a local installation of the j5 automated DNA assembly software package to function properly:
The current version of j5 works well with standalone BLAST 2.9.0+. Note that earlier versions of the standalone BLAST package (e.g. 2.2.21) have a different interface and will not be compatible with the current version of j5.